We thoroughly analyzed the status of Langerhans cells, LC precursor cells, and the LC precursor-recruiting factors in sun-protected breast skin of young (16-28 years old, N=20) and elderly (53-74 years old, N=21) women, using techniques such as multiple immunostaining*4.
*4 Multiple immunostaining: A technique of dyeing multiple target factors in different fluorescent colors in a single sample. This technique has an advantage in that correlation between multiple factors can be clearly determined.　

1. The number of epidermal Langerhans cells decreases with age
As in previous study results, it was confirmed that epidermal Langerhans cells were significantly reduced in aged skin (Figure 2).

2. The number of dermal LC precursor cells decreases with age
Next, we quantified the number of CD14, CD207 and CCR6-positive cells, which are considered LC precursor cells, and found that there was a significant decrease in the dermis of aged skin (Figure 3).

3. LC precursor-recruiting factor CXCL14 is down-regulated in epidermis with age
Furthermore, we examined the expression of factors that can recruit LC precursor cells to the epidermis, and among these found that the expression level of CXCL14 in keratinocytes was significantly reduced in aged skin (Figure 4).

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Shiseido Co. Ltd. published this content on 13 October 2020 and is solely responsible for the information contained therein. Distributed by Public, unedited and unaltered, on 13 October 2020 02:09:07 UTC