2019 AUG 14 (NewsRx) -- By a News Reporter-Staff News Editor at China Business Day -- Data detailed on Immunology - Antibodies have been presented. According to news reporting originating from Shanghai, People’s Republic of China, by NewsRx correspondents, research stated, “Despite the use of knobs-into-holes (KiH) strategy to promote heterodimerization in recombinant production of bispecific antibody (bsAb), homodimer (especially the hole-hole homodimer) can still be generated in small amount. This by-product needs to be removed by downstream process.”
Our news editors obtained a quote from the research from WuXi Biologics, “However, as homodimer and the target bsAb are usually very close in size, these two species may not be readily differentiated using size-exclusion chromatography-high performance liquid chromatography (SEC-HPLC). Thus, method other than SEC-HPLC needs to be developed to monitor removal of this by-product.”
According to the news editors, the research concluded: “Here, through a case study we demonstrate that analytical hydrophobic interaction chromatography (HIC) is a powerful tool for quantitatively monitoring removal of hole-hole homodimer in bsAb purification.”
For more information on this research see: Monitoring removal of hole-hole homodimer by analytical hydrophobic interaction chromatography in purifying a bispecific antibody. Protein Expression and Purification, 2019;164():105457. Protein Expression and Purification can be contacted at: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA. (Elsevier - www.elsevier.com; Protein Expression and Purification - http://www.journals.elsevier.com/protein-expression-and-purification/)
The news editors report that additional information may be obtained by contacting Y. Li, Technology and Process Development (TPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, People’s Republic of China. Additional authors for this research include J. Han, G. Guo, Q. Wang, Y. Wang and Y. Li.
The direct object identifier (DOI) for that additional information is: https://doi.org/10.1016/j.pep.2019.105457. This DOI is a link to an online electronic document that is either free or for purchase, and can be your direct source for a journal article and its citation.
Publisher contact information for the journal Protein Expression and Purification is: Academic Press Inc Elsevier Science, 525 B St, Ste 1900, San Diego, CA 92101-4495, USA.
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