Berkshire bought about 21.2 million Bank of America shares from July 28 to 30, according to a regulatory filing.
Following those purchases, Berkshire owned a total of 1.02 billion shares in Bank of America which roughly represents an 11.8% stake in the company.
Berkshire has spent more than $1.7 billion on 71.5 million Bank of America shares since July 20, including the latest purchases, according to regulatory filings.
Berkshire began investing in Bank of America in 2011, a time many investors worried about the bank's capital needs, when it acquired $5 billion of preferred stock plus warrants to buy 700 million common shares.
By the end of March, Bank of America had become Berkshire's second-largest common stock investment, trailing only Apple Inc . Berkshire also invests in several other banks.
The latest purchases help Omaha, Nebraska-based Berkshire reduce its cash pile, which totaled $137.2 billion at the end of March.
(Reporting by Jonathan Stempel in New York; Editing by Ana Nicolaci da Costa)