Uniqlo made a U-turn earlier in the day after CEO Tadashi Yanai had initially said the company planned to keep its stores there open, because "clothing is a necessity of life".
But, on Thursday, it said it could not continue business in the country because of "a number of difficulties".
Now, it - along with some of the latest names like Japan Tobacco - has joined a growing group of global brands shunning Russia after its invasion of Ukraine.
In a statement, Uniqlo parent Fast Retailing also condemned "all forms of aggression that violate human rights."
One Moscow resident voiced concern for those who will be out of work after the company's exit.
"It's sad that so many world brands are suspending their work in Russia. And it's too bad that so many people will be out of jobs. This situation is awful and I hope that soon everything will change and return to normal."
The exodus by Japanese companies in particular has picked up steam, with many describing the decisions in practical terms.
Among them, Toyota has cited logistical hurdles for suspending its operations in Russia, as more countries slap sanctions on Moscow and shippers suspend routes there.
Moscow, which calls the war in Ukraine a "special military operation," has warned it might nationalize idled foreign assets in retaliation against Western sanctions.
Russian President Vladimir Putin said that his country would emerge stronger and more independent from Western sanctions, which he said were inevitable.
