Mission Bio announced the first-time deployment of one single-cell transduction assay across gene therapy company AVROBIO's lysosomal disorder pipeline. The two companies collaborated to develop a precise, reproducible assay using Mission Bio's Tapestri® Platform to rapidly measure the percentage of transduced cells in samples composed of thousands of cells. AVROBIO is presenting data today on the use of this assay to determine percent transduction in autologous, lentiviral vector-mediated, genetically modified drug product at the 4th Annual Gene Therapy Analytical Development (GTAD) Summit.

Transduction percentage is a critical quality attribute for cell and gene therapy manufacturing, measuring the successful transduction of therapeutic genes into target cells. Assessing this in thousands of cells with the previous percent transduction assay could take months, starting with a protracted cell growth step before performing PCR-based measurements on each single colony. But with the Tapestri®-based assay, AVROBIO's scientists can analyze thousands of single cells without a clonal outgrowth step, increasing throughput and precision.

In a single high-throughput assay developed with the Tapestri® Platform, Mission Bio's biopharma partners can assess multiple characteristics of single gene-modified cells, including transduction efficiency, vector copy number, on- and off-target genome edits, zygosity, and aberrant translocations.