Les Echos reported on Wednesday that French banks BNP Paribas, Credit Agricole and Societe Generale were mulling a bid for Orange Bank.
The newspaper added that Spanish bank Santander and U.S. private equity fund Cerberus were also considering a bid.
"We are not interested in Orange," Botin said during the annual earnings press conference in Madrid.
(Reporting by Emma Pinedo; Editing by Jesus Aguado)