GenScript USA Inc. launched the GenWand Double-Stranded DNA (dsDNA) Service for the manufacture of CRISPR knock-in homology- directed repair (HDR) templates in T cell engineering. Closed-end dsDNA enables researchers to perform gene knock-in with higher efficiency and lower toxicity than traditional PCR methods and is ideally suited for large-scale screening and scale-up in cell and gene therapy. GenScript's GenWand dsDNA service manufactures covalently closed-end linear DNA to help increase the stability of dsDNA by limiting endonucleases' digestion of the dsDNA once entering cells. It also mitigates non-homologous end joining risk and increases knock-in accuracy when using CRISPR homology-directed repair machinery. Made via a proprietary production process, GenScript's dsDNA is available from micrograms to grams, and in 2-10 kb sequences. Various quality grades are available from research grade to basic GMP. This new service complements a growing portfolio of offerings for CRISPR/Cas genome editing, including the GenExact? ssDNA Service, and Phase Appropriate GMPsgRNA through its CRISPR Synthetic Guide RNA Services. In an ongoing collaboration with the University of California, San Francisco, GenScript has delivered an approximately 2.3Kb ssDNA for T cell engineering with non-viral genome targeting using long DNA sequences. The company delivers more than 10,000 sgRNA annually to clients globally and has manufactured more than 30+ phase appropriate GMP sgRNA batches for biopharma clients located in China.