relatively
low for
mammalian
cell use.
*FMv6* Nov 2019 WBC 60kb+ 15.5 FM
to 56 -21.1 temperature
protocol
changes
improved N50
& yield
results in
more usable
DNA, tuneable
N50/throughpu
t equation
for mammalian
cells - no
difference to
v5 for
bacteria.
*FMv7* - - - - Undisclosed
*FMv8* Oct 2020 Bacteria 45.8 25.6 Better cell
re-suspension
to improve
sequencing
yield further
(>20%) at
high N50
leads to high
DNA recovery,
larger volume
of bacteria
use possible.
More DNA per
run or more
DNA for more
runs.
*FMv8* Nov 2020 Bacteria 36.7 28.5 Optimised
Yield at high
N50. Total
raw data
yield is 30Gb
pre Q7 cut
off
FM = Fire Monkey. WBC = white blood cells. Source; RevoluGen internal records.
*Figure 3
Cheaper $/GB sequencing results from using Fire Monkey*
Link to graphic: Figure3.pdf (b3cnewswire.com) [3]
*Sequencing *Platform* *Data *N50 Read *Max *Throughput/ *Throughput/ *Cost/Gb
Technology* type* Length* Read flow cell flow cell ($/Gb)*
Leng (Gb) Mean* (Gb) Max*
th*
*Pacific Sequel II CLR 30-60 >200 50-100 160 13-26
Biosciences
(PacBio)*
*Oxford MinION Long 10-60 >1,0 2-20 30 50-500
Nanopore 00
Technologie
s (ONT)*
*Illumina NovaSeq Single-end 0.05-0.25 0.25 65-3,000 >3,000 10-35
(ILMN)* 6000
*Fire Sequel II CLR 25 n/a 139 n/a 9.4
Monkey use
+*
*Fire MinION Long 45.8 n/a 25.6 n/a 39.1
Monkey use
+*
*Fire MinION Long 36.7 n/a 28.5 n/a 35.1
Monkey use
+*
CLR = continuous long-read. Adapted from Logsdon _et al_ 2020 Long Read Human
Genome Sequencing and its Applications. Source; Nature Review Genetics (21) 597-614
plus RevoluGen data. All cost estimates exclude the cost of labour,
instrumentation, maintenance, and computer resources.
-Ends-
*About RevoluGen*
RevoluGen is a privately held scientific research and development company
commercialising molecular tools with a specific focus on rapidly extracting long
and pure DNA fragments from cells.
RevoluGen's Nucleic Acid Isolation and Purification (NAIP) products have particular
applications in long-read DNA sequencing for genome assembly and long-range PCR.
Fire Monkey significantly improves long-read sequencing results by extracting
long-fragment DNA with a simultaneous size selection function built into the
protocol that minimises the small-fragment DNA contamination. Used independently,
the size selection protocol of Fire Flower can improve the molecular ratio of any
extraction kit and is compatible with all sequencing technologies.
The Company headquarters, R&D, manufacturing and direct customer sales and support
are based in the UK. RevoluGen serves customers worldwide and has secured
agreements with world-leading molecular biology tools companies including Merck
KGaA (sales), Cytiva (manufacturing), Tecan (automation) and A4P (logistics).
For more information, please visit www.revolugen.co.uk [4]. *Follow us on *Twitter
[5] *and *LinkedIN [6]
*For more information or to arrange media interviews please contact:*
Sue Charles, Head Corporate Communications and Investor Relations, RevoluGen
+44 (0)7968 726585, sue.charles59@outlook.com
2020-11-30 Dissemination of a Corporate News, transmitted by DGAP - a service of
EQS Group AG.
The issuer is solely responsible for the content of this announcement.
The DGAP Distribution Services include Regulatory Announcements,
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