SYNLAB collaborated with researchers from the Universities of Heidelberg and Graz, and a large COVID-19 test centre in Stuttgart to conduct the COVID-19 Antigen Study (COVAG Study). The COVAG Study is one of the most comprehensive field research studies to systemically assess the actual sensitivity and specificity of rapid antigen tests. The results indicate that the actual sensitivity of two commonly used rapid antigen tests considered reliable, are only at 60.4 and 56.8%, respectively, compared to PCR tests. In addition, for the first time the COVAG Study identified a variant-dependency of rapid antigen tests: the sensitivity of the tests is significantly lower when detecting the alpha variant compared to their sensitivity when detecting the SARS-CoV-2 wild-type. 338 out of 2,215 individuals participating in the study tested positive for SARS-CoV-2 using the PCR testing method. Of these 338 positive cases, however, the two rapid antigen tests only identified 204 and 192 participants, respectively, as virus carriers. In other words, rapid antigen tests missed four out of ten individuals who tested positive for SARS-CoV-2 with PCR tests. Here, the viral load was the most decisive factor for the detection of the virus. Only individuals with a very high viral load (CT value <= 20) were reliably detected as virus carriers by rapid antigen tests. The virus, however, already spreads at a medium to low viral load. In addition, the COVAG Study was the first to investigate variant dependence of rapid antigen tests in samples with moderate to high viral loads (CT value <= 30). For the SARS-CoV-2 wild-type, the two rapid antigen tests' sensitivity was determined with 87.7 and 84.0%, respectively. Yet, for the alpha variant the same tests' sensitivity was only at 77.1 and 72.3%, respectively. Compared to other circulating variants, however, the alpha variant has the lowest structural deviation from the SARS-CoV-2 wild-type. Accordingly, it can be assumed that the sensitivity of rapid antigen tests is also significantly lower for other virus variants than for the original wild-type. Meanwhile, however, the latter has been completely displaced by the spread of new variants.