ProQR Therapeutics NV announced new preclinical data for its proprietary Axiomer? RNA editing technology platform, including the first preclinical proof of concept data for its AX-0810 pipeline program for cholestatic diseases targeting NTCP. The data are being presented at a poster session on May 8, 2024 at the American Society of Gene & Cell Therapy (ASGCT) Annual Meeting, May 7-11, 2024, in Baltimore, Maryland.

Preclinical proof of concept for AX-0810: AX-0810 is an editing oligonucleotide (EON) development program targeting the SLC10A1 RNA using the Axiomer technology, providing a transient and controlled approach that aims to reduce bile acids concentration in the liver. By specifically affecting the main transporter for bile acids reuptake from the portal vein circulation to the liver, called NTCP (Na-taurocholate transporting polypeptide, SLC10A1 gene), the AX-0810 program represents a promising avenue to ameliorate the progression of cholestatic disorders. As reported [ASGCT 2024, P-705], modulation of NTCP at different levels of editing leads to an increase in the biomarker Total Bile Acid in serum.

Data presented include: Axiomer EONs can specifically modulate NTCP protein bile acids reuptake function while preserving expression of the protein. A strong correlation (R2 = 0.51) was reported between editing levels of NTCP and bile acids change in the serum in NHP in vivo. An early generation of ProQR?s Axiomer editing oligonucleotides (EONs), EON1, yielded up to 29% editing of NTCP in the liver of non-human primates (NHPs) after a single dose and, importantly, this led to an 8-fold change in the serum biomarker bile acids 72 hours after treatment.

Further optimizations for EONs targeting NTCP have enabled achievement of up to 60% editing (in vitro). Results reported in NHPs confirmed findings in vitro models and showed translatability across models. ProQR is developing its AX-0810 program targeting NTCP for Cholestatic Diseases and plans to advance the program to the clinic in late 2024/early 2025.

ProQR also expects the following Axiomer pipeline program milestones in 2024: AX-0810 clinical development candidate translational data to be reported in H2 2024, with further detail on design for the clinical trial. AX-1412 preclinical proof of concept data and translational data to be reported in H2 2024, with program to advance to the clinic in late 2024/early 2025. AX-1412 is the Company?s Axiomer program targeting B4GALT1 for cardiovascular disease.