4basebio PLC announced the expansion of its synthetic DNA product offering. Following 4basebio's announcement in June 2021 regarding the development of its hpDNA™ and osDNA™ synthetic DNA constructs, 4basebio has now added opDNA™ and oeDNA™ to its product offering. This reflects the uniquely customisable characteristics of 4basebio's synthetic DNA technology.

The DNA product offering now comprises: hpDNA™ is a double stranded linear DNA, covalently closed with single strand hairpins at the 5' and 3' ends and ideally suited for viral and non-viral vector applications. osDNA™ incorporates nucleotide modifications within the DNA backbone providing resistance to exonuclease degradation, as well as tuning of the immunostimulatory properties of the construct, making osDNA™ ideally suited for DNA vaccine applications. opDNA™ is a partially opened, linear, double stranded DNA product.

Each opDNA™ molecule features a hairpin on either the 3' or 5' end, with an opposite open end. The open end of the construct can include an overhang or blunt end. This product is ideally suited as a template for IVT production of mRNA.

oeDNA™ is a linear open-ended, double stranded DNA product where both 3' and 5' ends are open. Constructs can be generated with blunt ends, overhangs or any combination thereof. Like all 4basebio's DNA products, oeDNA™ is resistant to degradation by exonuclease and hence suitable for in vivo applications.

This format is ideal for genome editing by homology directed repair (HDR).